《植物生理学报》 2013, 49(10): 1053-1056

苹果砧木‘青砧一号’离体高效再生体系的建立

吴瑞刚^1,2, 杨洪强^1,2,*, 沙广利³, 杨萍萍^1,2, 毕润霞^1,2

¹山东农业大学园艺科学与工程学院, 山东泰安271018; ²作物生物学国家重点实验室, 山东泰安271018; ³青岛市农业科学研究院果茶研究所, 山东青岛266100

通信作者：杨洪强；E-mail: hqyang@sdau.edu.cn, labft@sdau.edu.cn；Tel: 0538-8249304

摘　要：

‘青砧一号’是杂交育成的苹果矮化砧木新品系。本文探讨了影响‘青砧一号’离体再生的条件, 建立了高效离体再生体系。结果表明, 以叶片为外植体, 远轴面接触培养基, 最佳不定芽诱导分化培养基: MS+5.0 mg·L-1 6-BA+0.3 mg·L-1 NAA; 最佳继代增殖培养基: MS+1.0 mg·L-1 6-BA+0.1 mg·L-1 IAA+20 g·L-1蔗糖, 不定芽的增殖倍数为2.7; 最佳生根培养基: 1/2MS+1.0 mg·L-1 IAA+20 g·L-1蔗糖, 生根率达100%, 平均生根数达5.7条。炼苗后移入珍珠岩、蛭石、草炭等体积混合的基质中, 20 d后移栽成活率达60%。

关键词：苹果砧木; ‘青砧一号’; 不定芽分化; 再生

收稿：2013-06-21   修定：2013-07-15

资助：国家自然科学基金(31171923)、国家转基因生物新品种培育重大专项(2014ZX08009-3)和高校博士学科点专项科研基金(20103702110003)

Establishment of Efficient in vitro Regeneration System of Apple Rootstock ‘QZ-1’ (Malus hupehensis×M. domestica)

WU Rui-Gang^1,2, YANG Hong-Qiang^1,2,*, SHA Guang-Li³, YANG Ping-Ping^1,2, BI Run-Xia^1,2

¹College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, Shandong 271018, China; ²State Key Laboratory of Crop Biology, Tai’an, Shandong 271018, China; ³Fruit Institute, Qingdao Academy of Agricultural Sciences, Qingdao, Shandong 266100, China

Corresponding author: YANG Hong-Qiang; E-mail: hqyang@sdau.edu.cn, labft@sdau.edu.cn; Tel: 0538-8249304

Abstract:

‘QZ-1’ (Malus hupehensis×M. domestica) is a new strain of apple dwarfing rootstocks created by hybridization. In this experiment, the factors influencing leaf regeneration of ‘QZ-1’ were studied and the efficient in vitro regeneration system of ‘QZ-1’ was established. The results showed that MS medium with 0.3 mg·L^-1 NAA and 5.0 mg·L^-1 6-BA was optimal for adventitious bud differentiation. The best medium for subculture proliferation was MS supplemented with 1.0 mg·L^-1 6-BA, 0.1 mg·L^-1 IAA and 20 g·L^-1 sucrose, and the proliferation times of adventitious buds reached 2.7 on it. 1/2MS medium containing 1.0 mg·L^-1 IAA and 20 g·L^-1 sucrose was the most suitable for rooting. The rooting rate was up to 100%, and the average number of rooting reached 5.7. After acclimatization, the regenerated plants were transplanted into the matrix, which contained perlite, vermiculite and peat (1:1:1, V:V:V). The survival rate was up to 60% after 20 d.

Key words: apple rootstock; ‘QZ-1’; adventitious bud differentiation; regeneration